Pre-Setup Requirements
Before starting your calibration, prepare your equipment to ensure the most accurate readings:
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Calibrator Diagnostics: Run a quick leak test in the diagnostics menu of the BIOS Defender to confirm there are no internal leaks that could skew your results [00:32].
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Measurement Modes: Familiarize yourself with the BIOS Defender measurement modes: Single, Continuous, and Burst. The Continuous mode is often preferred for easy, ongoing readings during adjustment [01:23].
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Sample Train Setup: Always calibrate using the exact sample train (tubing, cassette, etc.) that will be used during actual sampling to account for any backpressure [02:57].
Step 1: Setting the Target Flow Rate
Establish your target flow on the pump before connecting it to the calibrator:
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Initial Setting: Power on the GilAir Plus and use the plus/minus buttons to set your desired flow rate (e.g., 2500 cc/min). Press the checkmark to save the target [02:22].
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Enter Calibration Mode: Navigate to the Calibrate menu on the pump to begin running the motor for calibration [02:48].
Step 2: Connecting and Measuring
Link the pump to the calibrator to view the actual flow being pulled:
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Connection: Connect the tubing from the pump's sample train to the Suction port on the BIOS Defender [03:52].
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Read the Calibrator: The BIOS Defender will display the real-time flow rate and a running average. Compare this average to your target setting on the pump [04:18].
Step 3: Adjusting and Finalizing
Match the pump's internal flow reading to the external calibration standard:
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Manual Adjustment: Use the plus and minus buttons on the pump to adjust the value displayed on its screen to match the average flow rate shown on the BIOS Defender [04:41].
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Confirm Adjustment: Once the pump's value matches the calibrator, press the checkmark. The pump will adjust its motor speed to reach the original target based on this new calibration point [05:00].
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Completion: When the average flow rate matches your target, press the checkmark one final time to complete the calibration [06:03].
